Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea resulting in high mortality in affected swine, particularly neonatal piglets, characterize PED disease. This emerging virus rapidly spreads and has caused significant economic losses, primarily due to very high mortality (50% to 100%) of young piglets in affected herds. The aim of this project is to create a sensitive new diagnostic tool that will be able to distinguish infectious viruses from non-infectious ones over a short period of time and be able to scan different sample types such as swabs from clinical cases, environmental samples and porcine-derived food additives like bones, meat, and blood meals.